The Lionesses suffered their first defeat under Sarina Wiegman when beaten 2-0 by Australia at the Brentford Community Stadium.
Australia became the first side to beat England since April 2021, ending a 30-game unbeaten run in front of a 17,000-strong crowd.
Hayley Raso saw her shot blocked before Sam Kerr pounced on Leah Williamson’s attempted headed back-pass to dink over Mary Earps.
England rallied with Georgia Stanway’s shot blocked and Chloe Kelly’s whipped cross only gathered at the second attempt by Mackenzie Arnold ahead of the onrushing Alessia Russo.
After the break Lauren James’ cross was sliced into the side netting by Clare Hunt, before James forced Arnold into a smart save.
Australia doubled their lead after 67 minutes on a swift counter-attack, Kerr crossing for Charlotte Grant to head home via a deflection off Williamson.
Rachel Daly put her free header from Lucy Bronze’s cross wide and Jess Park found Russo but her shot was blocked, as the Lionesses were unable to get back into the game.