Arsenal will begin their defence of the FA Girls Youth Cup away to Reading on Saturday 28 September.
Chelsea were beaten finalists last season and have an away tie at Charlton Athletic, Plate winners Doncaster Rovers Belles take on Manchester City.
MK Dons, who lost out to the Belles in the final, host Essex FA Regional Talent Centre, while there’s traditional rivalries as Everton meet Manchester United and Derby County face Nottingham Forest.
Saturday 28 September (11am)
Milton Keynes Dons v Essex RTC (Sir Herbert Leon Academy)
Charlton Athletic v Chelsea (CAFC Training Ground)
Bristol City v Southampton (SGS WISE Campus)
Birmingham City v Brighton & Hove Albion (Tally-Ho Police Sports Ground)
Wolverhampton Wanderers v Gillingham (Compton Park Training Ground)
Reading v Arsenal (Henwick Worthy Playing Ground)
Millwall Lionesses v Aston Villa (Ten Em Bee Sports Development Centre)
West Bromwich Albion v Oxford United (Aston University Recreation Centre)
Sunderland & Gateshead v Teesside Sports (Gateshead College, Academy for Sport)
Leeds United v Stoke City (Sport Park Weetwood)
Doncaster Rovers Belles v Manchester City (Keepmoat Stadium)
Everton v Manchester United (USM Finch Farm, Everton FC Academy)
Sheffield United v Blackburn Rovers (Manton Athletic Sports Club)
Leicester City v Liverpool (University of Leicester Pitches)
Durham v York City (Durham Racecourse)
Derby County v Nottingham Forest (University of Derby)